What Is A Template In Dna

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What Is A Template In Dna

A template strand is the term that refers to the strand used by DNA polymerase or RNA polymerase to attach complementary bases during DNA replication or RNA transcription, respectively; either molecule moves down the strand in the 3' to 5' direction, and at each subsequent base, it adds the complement of the current.
DNA is made up of a double helix of two complementary strands. During replication, these strands are separated. Each strand of the original DNA molecule then serves as a template for the production of its counterpart, a process referred to as semiconservative replication.
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Only then does the DNA polymerase link the incoming base with the predecessor in the chain. Thus, DNA polymerases are template-directed enzymes.
Curr Protoc Mol Biol. 2001 May;Chapter 7:Unit7.3. doi: 10.1002/0471142727.mb0703s21. Preparation of templates for DNA sequencing. Slatko BE(1), Heinrich .
The main enzyme involved in transcription is RNA polymerase, which uses a single-stranded DNA template to synthesize a complementary strand of RNA.

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